Competitive allele specifi c PCR (KASP): features, the interpretation of the results
Submitted by Гость (not verified) on Tue, 07/09/2024 - 16:45The use of competitive allele-specific PCR is becoming a popular method for mass crop genotyping. The development of markers for SNPs in polyploid organisms requires additional validation and testing when developing primers for further use. In this work, we propose an algorithm for testing and interpreting the performance of KASP markers on wheat using existing common wheat genomic assemblies. Using the described algorithm, six KASP-markers on VIVIPAROUS-1, MOTHER OF FT AND TFL1, TGW6-A1, PSY-A1, Dreb-B1 and leaf rust resistance locus Lr14a were analyzed.