Competitive allele specifi c PCR  (KASP): features, the interpretation of the results

Е. А. Никитина; А. В. Архипов; Я. В. Минькова; А. С. Яновский; В. А. Коробкова; М. А. Самарина; А. Г. Черноок; П. Ю. Крупин; Г. И. Карлов; М. Г. Дивашук

Submitted by Гость (not verified) on Tue, 07/09/2024 - 16:45

Известия Тимирязевской сельскохозяйственной академии. 6 / 2023

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Е. А. Никитина cover

А. В. Архипов cover

Я. В. Минькова cover

А. С. Яновский cover

В. А. Коробкова cover

М. А. Самарина cover

А. Г. Черноок cover

П. Ю. Крупин cover

Г. И. Карлов cover

М. Г. Дивашук

The use of competitive allele-specific PCR is becoming a popular method for mass crop genotyping. The development of markers for SNPs in polyploid organisms requires additional validation and testing when developing primers for further use. In this work, we propose an algorithm for testing and interpreting the performance of KASP markers on wheat using existing common wheat genomic assemblies. Using the described algorithm, six KASP-markers on VIVIPAROUS-1, MOTHER OF FT AND TFL1, TGW6-A1, PSY-A1, Dreb-B1 and leaf rust resistance locus Lr14a were analyzed. The most promising KASP markers by bioinformatic analysis and PCR were the markers for VIVIPAROUS-1, MOTHER OF FT AND TFL1 genes.

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