The article presents the results of research on the adaptability of planting material of neglected sweetvetch (Hedysarum neglectum Ledeb.) obtained by clonal micropropagation in the natural and climatic conditions of the Vologda District, Vologda Region, Russia. Currently, the development of the medicinal plant industry in Russia plays an important role within the concept of saving the health of the population. Industrial cultivation of rare medicinal plants using modern adaptive cultivation technologies is important for organic agriculture and the conservation of the biodiversity of phytogenic resources. H. neglectum is the most important herbaceous plant with a wide range of pharmacological activity. The winter hardiness of the cultivated H. neglectum plants in the experimental area after the 1st overwintering was 90%, after the 2nd overwintering – 100%. 3-year-old H. neglectum plants formed three times more erect leafy shoots than 2-year-old plants. Mass maturation of seeds of 2–3-year-old H. neglectum plants in the Vologda Region is observed at the end of the 1st decade of August. The height of 2–3-year-old H. neglectum plants was 55.0–59.9 cm, while 3-year-old plants formed an average of 5.6 generative shoots per plant, which is three times more generative shoots compared to 2-year-old plants. The mass of the above-ground part of one shoot of 3-year-old H. neglectum plants averaged 20.9 g, which is 50% more than that of 2-year-old plants. The yield of H. neglectum phytomass of above-ground part in the air-dry state in 3-year-old plants was 117 g/m2, which is five times more compared to 2-year-old plants. A sufficiently high adaptability of 3-year-old H. neglectum plants to the agro-climatic conditions of the Vologda Region was noted.