Currently, there are no scientifically approved resource-saving technologies for growing plum seedlings in Non-Chernozem nurseries. Therefore, the study was aimed at determining the effect of variable soil moisture supply on the growth and development of plum seedlings. Based on this parameter, a rational mosture regime of Non-Chernozem nurseries can be developed. Field studies were conducted on the territory of a study experimental farm and the fruit-growing laboratory “Mi-churinsky Garden” at Russian Timiryazev State Agrarian University. Two-factor experiment to study the effect of different mosture regimes on the development of plum seedlings of two varieties grafted on plum seedling rootstock was laid in the spring of 2016. A long-lasting drip line was used for irrigation. Soil moisture was monitored using tensiometers graduated on the basis of the thermostat-weight data. The irrigation regime (irrigation frequency and rate) was selected based on the actual soil moisture, which is associated with such climatic indicators as air temperature and precipitation rate. The maximum irrigation rates are typical for the majority of moistened options, but it should be noted that the irrigation rate in 2017 was greater than that of 2016. The experimental results have shown that plum seedlings grown under conditions of insufficient and uneven moisture supply are characterized by the lowest biometric indicators. The biometric indicators of plants prove that the most optimal is the drip irrigation regime with maintaining soil moisture in the range of80-100% HB.