Currently, a priority trend in poultry science is improving the energy efficiency of poultry production. To achieve this goal, it is necessary, in particular, to develop and implement energy-saving technologies such as improving ventilation and heating systems to ensure air exchange and heating, enhancing microclimate control elements, and maintaining an optimal microclimate environment in production premises. The research was conducted in order to scientifically substantiate the need for microclimate uniformity control in poultry houses. Weekly monitoring of air flow distribution uniformity in broiler rearing facilities became possible with the development of the microclimate uniformity index, calculated on the basis of carbon dioxide concentration values in different parts and zones of the poultry house. Broilers were reared in six different zones of the facility in battery cages. It was established that under broiler rearing conditions accounting for microclimate uniformity in production premises, the zootechnical efficiency is characterized by an increase in average live weight of broilers reared in the central part of the poultry house by 3.9%, poultry survival rate by 2.9–4.4%, a reduction in feed costs per 1 kg of live weight gain by 0.04 kg, and an increase in the broiler productivity index by 18–39 units compared to values obtained when rearing broilers in other parts of the poultry house. At the same time, the following microclimate parameter values in the air environment in the bird housing zone for the pre-slaughter period (35 days and older) should be maintained: temperature – not below 19.0°C; relative humidity – not above 57.0%; carbon dioxide concentration – not above 2700 ppm; ammonia concentration – not above 3.5 mg/m3; microclimate uniformity index – not below 2.7 units.