Е. А. Исакова The feasibility of using the liposomal preparation (LP) with encapsulated ATP for subclinical mastitis in cows during the peak lactation period is scientifically substantiated. Twenty cows with subclinical mastitis were selected for the experiment by the diagnostic product “Masttest”. The cows were similar in the following characteristics: breed – Holstein Black-and-White; weight – 400–415 kg; age – 2–3 years. The 20 cows with subclinical mastitis were divided into two groups of 10 animals: experimental and control. Animals in the experimental group received 10 ml of the liposomal preparation (LP) with encapsulated ATP intramuscularly into the croup, while animals in the control group received the same dose of sterile isotonic sodium chloride solution intramuscularly into the croup. The preparation was administered daily for 10 days. Two days after the last injection, a repeated somatic cell count analysis of milk was performed using Masttest, which showed that subclinical mastitis persisted. Using enzyme-linked immunosorbent assay (ELISA) of cow blood serum, it was found that the cause of subclinical mastitis in cows was Staphylococcus, the concentration of which decreased after the liposomal treatment course in the experimental group compared to the control. By the end of the experiment biochemical parameters revealed a significant increase in uric acid (UA) concentrations in both the experimental and control groups. No significant changes in hematological parameters were observed between the experimental and control groups. Thus, the experiment established that the liposomal preparation with encapsulated ATP reduces bacterial load and exhibits antioxidant effects; however, it does not ensure complete restoration of mammary tissue at a dosage of 10 ml per day for 10 days. The data obtained confirm the need for further research to develop more effective liposomal agents with targeted delivery of active substances and methods for regulating the immune response to mastitis in cows.
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