The aim of the research is to study the effects of amino acid-based and seaweed-based fertilizers on the linear and physiological parameters of different garden iris varieties (Cherry Garden, Lace Caper, Pink Pincurls). The experiment utilized Beres brand preparations (BeresAminoComplex and Beres Super Extract of Seaweed). The field experiment was carried out on the experimental plot of the South-Ural Botanical Garden-Institute of Ufa Federal Research Center of the Russian Academy of Sciences in 2022–2023 (Bashkortostan, Ufa) in the following variants: control, fertigation, foliar treatment, a combination of fertigation and foliar spraying. The study evaluated linear biomorphological parameters of plant organs, including flower diameter and height, length and width of upper and lower perianth lobes, leaf length and width, and generative shoot height and width. Water regime parameters were also assessed, encompassing total water content, water holding capacity, and water deficit. The results indicated that agrostimulation with the seaweedbased preparation was more effective than that with the amino acid-based preparation. Specifically, the seaweed treatment resulted in a 7–17.2% increase in 11 out of 60 linear indicators, while the amino acid treatment increased 3 linear indicators by 6–33%. The treatment methods also influenced plant growth differently. Algae extract application led to a slight increase in water-holding capacity, whereas the amino acid treatments showed no change in this parameter. Both preparation types were most effective in reducing water deficit, with decreases ranging from 17.9 to 43.1%.