О. С. Шаталина Immunogenetic evaluation is an important component of appropriate breeding and selection work. It allows keeping rigorous records of the origin authenticity and identify genotypes linked to productivity, as well as to breed cattle with desired or predicted parameters. It is impossible to perform blood grouping without specific reagents (monospecific sera), and their obtaining is important work conducted by the specialists of the Immunogenetic Evaluation Laboratory at the Ural Research Institute of Agriculture. Due to the decrease in the number and volume of reagents, it became necessary to produce them using the laboratory internal resources. Thus, the purpose of this work is to replenish the reagent bank with the required monospecific sera. In order to obtain monospecific sera, the authors used a method of cattle immunization with the selection of donor-recipient pairs. Upon sampling and selection procedures, 100 ml of blood was once taken from donor bulls and then admixed to recipient bulls in three steps 30 ml each time at an interval of one week. Donor herds of Ural-type cows and fattening bulls were immunized at agricultural breeding enterprises located in the Sverdlovsk Region: LLC Mezenskoye, Pervouralsk Integrated Agricultural Production Center, LLC Borodulinskoye, Integrated Agricultural Production Center - Kolkhoz named after Sverdlov, Patrushi Agricultural Company, After the immunization, serum is obtained from the blood of recipient bulls and analyzed with the method of dilution for titre and specificity. There are three ways to obtain monospecific sera: immunization, absorption, and elution. On the basis of this methodology, 41 reagents were produced at the Immunogenetic Evaluation Laboratory between 2008 and 2017, the titres of which varied from native (1:1) to 1:512.
Научная статья 
monospecific sera