The activity of membrane enzyme systems (ionic pumps), ATPases being their integral proteins, is one of the sensitive criteria for metabolic processes in animals. In particular, physiological and biochemical processes occurring in erythrocytes and secretory cells of the mammary glands during milk formation are not an expetion here. The authors have conducted studies on lambs and ewes of Teksel and Kuibyshevskaya breed to identify the age and breed characteristics of the functioning of ATPases of the red blood cell membrane and the membrane of milk fat globules. The results have shown that the activity of Mg 2 +, Na +, K+-, Mg 2 +- and Na +, K+-ATPase membrane erythrocytes of lambs are determined by age with a high degree of confidence (P 2 +- and Na+, K+-ATPases of the membrane of ewe’s milk fat globules - by 87.15%; 89.36 and 88.09%, respectively. No significant influence of the species of animals, as well as no joint influence of age and breed factors on the activity of all the studied ATPases of the erythrocyte and milk membrane components has been observed. The results of correlation and dispersion analyzes proved a significant effect on the ATPase activity of blood and milk on the age and species of animals; it has been proved that the activity of total, Mg 2 +- and Na +, K+-ATPases of the erythrocyte membrane is reliably (P < 0.001) determined by the species of animals by 98.83%, 99.45%, and 96.14%, respectively; the activity of the ATPase membrane of milk fat globules - by 95.57%; 92.09% and 82.27%, respectively.