One of modern agronomical soil science priorities is the study of soil and biotic transformation processes of materials used as alternative fertilizers, with subsequent assessment of changes both in the nutritious quality of a soil solution composition and in shift directions of a function of the soil-biotic trasformation. In this regard, the present work is devoted to studying the microbiological characteristics of sod-podzolic sandy loamy soil and, in particular, the biochemical function of the transformation of its organic and mineral phosphate components occurring under the influence of zeolite from the Hotynetsky field. There has been conducted a microfield research of the influence of high rates of zeolite on the quantity indicators of litothrophic and organothrophic phosphate-reduction microorganisms of soil and its phosphatase activity. The research has shown that the quantity of geophilic litothrophic microorganisms under the influence of zeolite at a rate of 6 t/hectare increased on average in 2.6-3.0 times, and that of organothrophic microorganisms - by 80%. The frequency rate of strengthening the soil phosphatase activity by the rock material at rates of 6 and 12 t/hectare, amounted to 3.0 and 3.3 times, respectively. Owing to the activization of phosphate-reduction microbic associations of sod-podzolic soil with high-siliceous material, the content of mobile phosphorus compounds increased from 22% to 43%, depending on a zeolite rate. The close and prolonged by years of research correlation between the content of mobile phosphates in soil and the number ofphosphate reduction microorganisms convincingly confirms the fact of active interaction of the considered rock material with a soil-and-biotic complex and, at the same time, allows to consider it as a material stabilizing not only biodegradation functions of a micro-bic pool, but also optimizing the soil absorbing complex due to exchange reactions in colloidal Si-P system.