The article presents the results of studies on phenological and morphological characteristics of forest berry plants obtained by microclonal propagation and adapted ex vitro, after transplanting to open ground conditions in the Vologda district of the Vologda region. Nowadays, in the conditions of import substitution and demand for fruit and berry products, the industrial cultivation of berry planting material is necessary. The objects of research are lingonberry (Vaccinium vitis-idaea L.) cultivars ‘Kostromichka’ and ‘Kostromskaya Rozovaya’, lowbush blueberry (Vaccinium angustifolium Ait.) cultivars ‘Northblue’ and ‘Northcountry’, European cranberry (Vaccinium oxycoccos L.) cultivars ‘Dar Kostromy’ and ‘Severyanka’, arctic bramble (Rubus arcticus L.) cultivars ‘Astra’ and ‘Galina’. The winter hardiness of 2-year-old seedlings of the studied berry cultivars obtained by the in vitro method after overwintering was 89–100% in the first decade of May. The average height of 2-year-old R. arcticus plants is 9.3 to 10.2 cm, V. angustifolium – 17.4 to 18.1 cm, V. oxococcos – 6.4 to 7.2 cm, V. vitis-idaea – 6.2 to 7.0 cm. The yield of air-dry phytomass of leaves of 2-year-old R. arcticus plants averaged 112.9 to 151.8 g/m2, V. vitis-idaea – 2.1 to 2.2 g/m2; the air-dry mass of plant leaves was 18 to 21% of the wet mass. Leaf damage by the cruciferous flea beetle (Phyllotreta Stephens) was observed on 2-year-old R. arcticus plants. The results obtained indicate a rather high adaptability of the studied berry plants to the soil and climatic conditions of the Vologda region, Russia.