S with alfalfa varia were carried out under environmental conditions of the premountain zone of North Ossetia-Alania on the black soil of peeled pebbles. The authors compared the effect of biological and mineral forms of nitrogen on the formation of yield and protein productivity of alfalfa crops. In the considered three-year study, eight full-fledged alfalfa harvests were obtained - two in the year of sowing and three in the following years. During the whole period, the control variant yielded 13.38 t/ha of hay. The industrial strain rhizo-bium 425a contributed to crop growth of 1.57 t/ha or 11.7%. The largest harvest was formed in crops with the highest level of symbiotic activity (In-1800) and amounted to 16.01 t/ha, which is 19.7% higher than the control variant. The protein productivity of alfalfa depended mainly on the activity of the symbiotic system and the mode of a nitrogen diet. The protein productivity for the entire study period was 2186 kg/ha in the control variant. The efficiency of starting doses of nitrogen fertilizers was only 1.6%. The combination of starting doses of fertilizer and active rhizobium strains increased protein productivity by 538 kg/ha or 24.6%. The maximum protein productivity was obtained in the variant with inoculation of seeds with active high-mountain strains of tuber bacteria and amounted to 2779 kg/ha, which is 27.2% more than in the control variant. On average, over three years, each harvest yielded 1.67 t/ha of hay in the control variant, which was equivalent to about 273 kg of protein. The maximum positive effect was obtained in the In-1800 variant: the average hay yield was 2.0 t/ha, and the protein productivity reached 347 kg/ha, which is 19.5 and 27.2% higher than the control variant, respectively.