The ampelographic collection of the Anapa Zonal Experimental Station of Viticulture and Winemaking is a research platform for breeding work. It consists of a large number of varieties of different ecological and geographical origin. At present, the gene pool of varieties in the collection consists of 4964 varieties, and it is replenished every year. These varieties and hybrids are used in the breeding process to create new, high-quality varieties of table and wine grapes. For successful breeding work in the ampelographic collection of the Anapa Zonal Experimental Station of Viticulture and Winemaking, variety studies are conducted to find new donors and sources of breeding-valuable traits, as well as resistance to biotic and abiotic environmental factors. This article presents the main results of the long-term work carried out by scientists-breeders at the Experimental Station to study and identify donors and sources of resistance to phylloxera, the main pest of grapes. The objects of research were the donor grape varieties of the selection of the Anapa Zonal Experimental Station of Viticulture and Winemaking, tolerant to phylloxera, as well as the source varieties of phylloxera resistance from various geographical zones. Modern programmes and methods were used in this research, in addition to traditional breeding techniques. The article presents two donors and six sources of phylloxera resistance, gives their brief characterisation, shows the dynamics of the main agrobiological and biochemical indicators for 2019–2021. Over the past three years, the breeders of the Anapa experimental Station have received more than 700 grapevine seedlings of a new hybrid generation, the fourth part of which are parental donors and sources of the varieties studied in this work.