The authors have experimentally established a possibility of penetration and development of entomopathogenic nematodes (EPN) in the corpses of insects killed not only by the nematodes themselves, but also under the influence of, insecticides, for example, negative temperatures, or other insect pathogens. This fact indicates the need to study the biology of these organisms to ensure objective assessment of the prospects of their effective practical use in agriculture. As a result of the conducted research it has been established that in the presence of live insects or their corpses in a certain amount of the soil, only a certain number of the nematodes which are in a zone of host dwelling get into the insect. As population density of infective larvae increases above the optimum, the laws of self-regulation begin to act on the micropopulation level, causing the most of the infective larvae flow into inactivated state and serve as the basis for the continued existence of EPN micropopulation in this biotope.